Comparative Studies on S-Glycoproteins Purified from Different S-Genotypes in Self-Incompatible BRASSICA Species II. Immunological Specificities.
نویسندگان
چکیده
Antisera were prepared by immunization of apparently purified S-glycoproteins; one from an S allele of Brassica campestris and two from S alleles of B. oleracea. Each antiserum was reactive not only with the homologous S-glycoprotein but also with the heterologous ones, i.e. with the S-glycoproteins of the other S alleles of the same locus. In double diffusion tests, a spur against the heterologous S-glycoproteins suggested heterogeneity of the glycoproteins. The heterogeneity appears to involve a component of the molecule in which the genotypic specificity of an S-glycoprotein resides, probably, for the recognition site. Some molecular components are common to all tested S-glycoproteins and in this respect are like the public antigens of the MHC locus of mammals. The common molecular components were recognized between the S-allele-specific glycoproteins within B. oleracea and also between them and those of B. campestris. No S-specific substances were detected in buffer soluble homogenates of style, ovary or anther. However, these homogenates contained substances that had structures similar to the corresponding common parts of the S-glycoproteins.
منابع مشابه
Comparative Studies on S-Glycoproteins Purified from Different S-Genotypes in Self-Incompatible BRASSICA Species I. Purification and Chemical Properties.
S-glycoproteins, i.e. stigma glycoproteins that are heritable in correlation with S allele in self-incompatible Brassica species, were apparently purified for three S alleles in B. oleracea. From SDS gel electrophoresis, the estimated molecular weight for two of the S-glycoproteins was 57,000. The other S-glycoprotein was considered to be heterogeneous with molecular weights of 60,000 and 65,00...
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عنوان ژورنال:
- Genetics
دوره 100 4 شماره
صفحات -
تاریخ انتشار 1982